Fig. 5
From: Advanced epithelial lung and gut barrier models demonstrate passage of microplastic particles
Permeability of fluorescent MNP. A-B MucilAir™ systems were apically exposed to PS-F 0.05 (1 mg/mL) for 24 or 96 h (2 independent experiments). PS-F 0.05 dose solution was replaced after 48 h. A Apical to basolateral permeability of PS-F 0.05 between 0 and 24 h (experiment 1, n = 6) or 0–48 and 48–96 h (experiment 2, n = 3). B Representative confocal microscopy images of blanc control and 96 h PS-F 0.05 exposed MucilAir™ systems. Dark blue: cell nuclei (DAPI); red: actin (phalloidin; cytoskeleton); light blue: cilia (acetylated-tubulin); green: MNP. Each staining is separately visualized with a specific laser in the blue, green, red or far-red channel, followed by projecting the signals from the separate channels in an overlay picture. C Apical to basolateral permeability of PS-F 0.05, PS-F 1 and PS-F 10 (all 1 mg/mL) in porcine jejunum, ileum and colon tissue explants mounted in the InTESTine™ after 5 h (n = 4). D Apical to basolateral permeability of PS-F 10 (1 mg/mL) in porcine colon tissue explants in the IEBC after 24 h (n = 4). E-G Representative confocal microscopy images of blanc control and 5 h PS-F 1 or PS-F 10 exposed porcine jejunum tissue explants. Dark blue: cell nuclei (DAPI); red: actin (phalloidin; cytoskeleton, intestinal/crypt linings); purple: cilia (acetylated-tubulin; cytoskeleton); light blue: MNP. Each staining is separately visualized with a specific laser in the blue, green, red or far-red channel, followed by projecting the signals from the separate channels in an overlay picture. E Jejunum tissue was imaged using a 10x objective. As the complete intestinal tissue segment (from apical to basolateral) did not fit in one field of view (FOV) using the 10x objective, 2 or 3 FOVs per slide were taken and combined into one image. F Jejunum tissue exposed to PS-F 1 and PS-F 10 was imaged using a 40x and 100x objective. G Z-stack: two orthogonal representations of jejunum tissue exposed to PS-F 1 or PS-F 10 showing that the plastic particles are at the same Z-position as the cells. Data are presented as mean ± SD (graphs) or as representative images