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Table 2 Acute ecotoxicity of microplastic particles towards D. magna in the 48 h immobilisation assay

From: Polymers of low concern? Assessment of microplastic particles used in 3D printing regarding their toxicity on Raphidocelis subcapitata and Daphnia magna

Application / Purpose

Polymer

Hypotheses for testing

Uptake

EC50 48 h

Result

Selective Laser Sintering (SLS) and variations of the same polymer backbones

TPU_ester_arom

• Thermoplastic vs. cross-linked

• Ester vs. Ether backbone

• aromatic vs. aliphatic soft segment

• foam vs. solid

Yes

> 1 g/L

No impact of linking, backbone, aromaticity, reactivity and state on D. magna toxicity

TPU_ether_arom

Yes

> 1 g/L

TPU_ester_aliph

Yes

> 1 g/L

TPU_ether_aliph

Yes

> 1 g/L

PU_binder_aromatic 1 C

Yes

> 1 g/L

PU_binder_aromatic 2 C

Yes

> 1 g/L

PU Foam

Yes

> 1 g/L

PA-6

• Size small vs. large

Yes

> 1 g/L

Size distribution had no impact on D. magna toxicity

PA-6 inhalable

Yes

~ 1 g/L

PA-12

Yes

> 1 g/L

Benchmark

HDPE_broad_distribution

• Size small vs. large

• branching (LDPE vs. HDPE)

Yes

~ 1 g/L

Different particle size and branching had no impact on D. magna toxicity

LDPE 250

Yes

> 1 g/L

LDPE 80

Yes

> 1 g/L

PMMA (monodisperse)

• Broad size distribution vs. monodispers

Yes

> 1 g/L

Size distribution had no impact on D. magna toxicity

PMMA_broad_distribution

Yes

> 1 g/L

Micronized Tire rubber

• Complex composition of additives (recycled tire)

Yes

> 1 g/L

Additive content had no impact on D. magna toxicity