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Table 2 Results of utilized linear models to analyse the microplastic concentration in fish, as well as the performance parameters, health parameters and gene expression, obtained from the exposure experiment. n = number of observations. df = degrees of freedom

From: Combined proteomic and gene expression analysis to investigate reduced performance in rainbow trout (Oncorhynchus mykiss) caused by environmentally relevant microplastic exposure

Parameter n df Model effect F-value p-value
Microplastic concentration 90 5, 84   0.5113a < 0.0001*
    Exposure level 54.863 < 0.0001*
    Pellet size 0.0 1.0000
    Exposure level x pellet size 2.0846 0.1312
Start weight 900 2, 897   0.1073 0.8983
End weight 374 2, 371   4.2307 0.0152*
    Exposure level 8.2067 0.0044*
Specific growth rate (SGR) 9 2, 6   4.9084 0.0546
    Exposure level 9.7445 0.0205*
Food conversion rate (FCR) 9 2, 6   6.41268 0.0232*
    Exposure level 88,148 0.0250*
Mortality 9 2, 6   0.8571 0.4705
Blood glucose level 315 6, 308   0.1033a 0.0201*
    Exposure level 1.1565 0.3243
    Sampling time 4.2788 0.0394*
    Fish total length 0.6181 0.4324
    Exposure level
x fish total length
2.1262 0.1213
Hematocrit 315 6, 308   -0.1403a 0.2310
    Exposure level 0.6617 0.5212
    Sampling time 0.0264 0.8710
    Fish total length 0.0580 0.8099
    Exposure level
x fish total length
1.0487 0.3518
Hepatosomatic Index 315 6, 308   0.3366a < 0.0001*
    Exposure level 0.1808 0.8352
    Sampling time 0.4921 0.4835
    Fish total length 8.3918 0.0040*
    Exposure level
x fish total length
0.9471 0.3891
TBARS Assay (liver) 133 4, 128   0.0945a 0.0914
    Exposure level 0.0919 0.7707
    Sampling time 7.8470 0.0059*
    Fish total length 8.0589 0.0053*
    Exposure level
x fish total length
0.0156 0.9008
TBARS Assay (intestine)     0.2483 0.0010*
    Exposure level 0.2423 0.6374
    Sampling time 8.5565 0.0041*
    Fish total length 4.5318 0.0352*
    Exposure level
x fish total length
0.1893 0.6642
  1. a = adjusted r2-value. * = statistically significant differences (p < 0.05)