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Table 2 Results of utilized linear models to analyse the microplastic concentration in fish, as well as the performance parameters, health parameters and gene expression, obtained from the exposure experiment. n = number of observations. df = degrees of freedom

From: Combined proteomic and gene expression analysis to investigate reduced performance in rainbow trout (Oncorhynchus mykiss) caused by environmentally relevant microplastic exposure

Parameter

n

df

Model effect

F-value

p-value

Microplastic concentration

90

5, 84

 

0.5113a

< 0.0001*

   

Exposure level

54.863

< 0.0001*

   

Pellet size

0.0

1.0000

   

Exposure level x pellet size

2.0846

0.1312

Start weight

900

2, 897

 

0.1073

0.8983

End weight

374

2, 371

 

4.2307

0.0152*

   

Exposure level

8.2067

0.0044*

Specific growth rate (SGR)

9

2, 6

 

4.9084

0.0546

   

Exposure level

9.7445

0.0205*

Food conversion rate (FCR)

9

2, 6

 

6.41268

0.0232*

   

Exposure level

88,148

0.0250*

Mortality

9

2, 6

 

0.8571

0.4705

Blood glucose level

315

6, 308

 

0.1033a

0.0201*

   

Exposure level

1.1565

0.3243

   

Sampling time

4.2788

0.0394*

   

Fish total length

0.6181

0.4324

   

Exposure level

x fish total length

2.1262

0.1213

Hematocrit

315

6, 308

 

-0.1403a

0.2310

   

Exposure level

0.6617

0.5212

   

Sampling time

0.0264

0.8710

   

Fish total length

0.0580

0.8099

   

Exposure level

x fish total length

1.0487

0.3518

Hepatosomatic Index

315

6, 308

 

0.3366a

< 0.0001*

   

Exposure level

0.1808

0.8352

   

Sampling time

0.4921

0.4835

   

Fish total length

8.3918

0.0040*

   

Exposure level

x fish total length

0.9471

0.3891

TBARS Assay (liver)

133

4, 128

 

0.0945a

0.0914

   

Exposure level

0.0919

0.7707

   

Sampling time

7.8470

0.0059*

   

Fish total length

8.0589

0.0053*

   

Exposure level

x fish total length

0.0156

0.9008

TBARS Assay (intestine)

   

0.2483

0.0010*

   

Exposure level

0.2423

0.6374

   

Sampling time

8.5565

0.0041*

   

Fish total length

4.5318

0.0352*

   

Exposure level

x fish total length

0.1893

0.6642

  1. a = adjusted r2-value. * = statistically significant differences (p < 0.05)