Fig. 3
From: Advanced epithelial lung and gut barrier models demonstrate passage of microplastic particles
Viability and functionality of human and porcine intestinal tissue explants upon exposure to MNP (1 mg/mL). A-B Human colon tissue explants were mounted in the InTESTine™ and exposed to MNP for 4 h (n = 3). A Cumulative LDH release into the apical and basolateral compartment. B Ratio of caffeine/mannitol (both applied at 10 μM) apparent permeability (Papp), calculated between 1 and 2 and 2–4 h of incubation. Cutoff for proper tissue functionality is 2, indicated by the dotted line. C Cumulative LDH release into the apical and basolateral compartment of porcine jejunum, ileum and colon tissue explants mounted in the InTESTine™ and exposed to MNP for 5 h (n = 4). D-E Control and PS-F 10 exposed porcine colon tissue explants in the Intestinal Explant Barrier Chip (IEBC) for 24 h (n = 3–4). D Cumulative LDH release into the apical and basolateral compartment. E Ratio of caffeine/mannitol (both applied at 10 μM) Papp, calculated between 3 and 5 and 22–24 h of incubation. Cutoff for proper tissue functionality is 2, indicated by the dotted line. Data are presented as mean ± SD. * p < 0.05 compared to the control condition, calculated by one-way ANOVA (Dunnett’s multiple comparisons)