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Fig. 2 | Microplastics and Nanoplastics

Fig. 2

From: Advanced epithelial lung and gut barrier models demonstrate passage of microplastic particles

Fig. 2

Viability and integrity measurements in the lung epithelial cell model MucilAir™ upon 24, 48, and 96 h exposure to MNP (1 mg/mL). A The release of lactate dehydrogenase (LDH) into the basolateral compartment was measured as a marker of cell viability. Maximum LDH release was achieved by exposure to Triton X-100 0.1%, and all other values were expressed as percentage relative to the maximum release. B Transepithelial electrical resistance (TEER) as marker for barrier integrity was measured before (t = 0 h) and after exposure. Per condition, TEER at t = 0 h was normalized to 1 and subsequent measurements were expressed relative to t = 0 h. Additionally, at every timepoint, ctrl TEER was set at 100%, and all other values were expressed as percentage relative to the ctrl. Inset: relative TEER under control conditions over time. TEER at t = 0 h was normalized to 1 (dotted line) and subsequent measurements were expressed relative to t = 0 h. Data are presented as mean ± SD. * p < 0.05 compared to the control condition, calculated by one-way ANOVA (Dunnett’s multiple comparisons). N.D. = not determined

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