Fig. 2

AF4 fractograms for three digested-filtered C. Robusta incubated with 100 nm PS and the associated control sans PS. The solid lines correspond to the MALS signal at 90°, the dashed lines correspond to the UV absorbance at 254 nm. The scatter plots (green and black) correspond, respectively, to the RMS diameter and the hydrodynamic diameter of the particles. On fractogram A, a dotted line is superimposed that represents the 100 nm PS peak measured separately (not from C. Robusta exposure); this data was used to define the elution range for collection of fraction 2 in samples a, b and c, which have increasing PS exposure levels